Resumen
The phasor plot approach is a method developed to analyze and interpret fluorescence lifetime imaging microscopy (FLIM) and hyperspectral imaging (HSI) without the need for data fitting or demixing models. Traditionally, the analysis of FLIM or HSI requires extensive knowledge and expertise to select the right model. These methodological and technical difficulties have limited the adoption of these advanced imaging technologies by only a few groups or centers worldwide, compromising their impact in many life science fields. Based on its intuitiveness and simple rules, the phasor approach has risen as a simple method to expand the access and utilization of these advanced imaging technologies. In this lecture, I will cover the phasor plots fundamentals for FLIM and HSI data, highlighted by some applications by several biophysical problems studies at the Advanced Biomiaging Unit (UBA, Institut Pasteur of Montevideo and the Universidad de la Republica, Uruguay). Finally, I will share the technological developments at the UBA, such as hardware, software, and methods, and the many opportunities for regional students and core facility staff to research and visit our center.